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C5122
mCS20ABN Medium
CS20ABN has been developed by Chang et al. to isolate Xanthomonas campestris pv. campestris (Xcc) from crucifer seeds. The original medium recipe allowed the quick isolation of most isolates of Xcc. However, the recovery of some isolates of Xcc was poor due to pH-dependent sensitivity to neomycin. In the modified version, the pH is lowered to 6.5 by the addi-tion of extra potassium dihydrogen phosphate. This modification improved the recovery of some neomycin-sensitive isolates considerably.Contaminated seed lots can be detected by dilution plating of the bacterial extract on mCS20ABN and mFS. Suspected isolates are then transferred to YDC. Finally, the identity of the suspected isolates can be determined by a pathogenicity test using brassica seedlings.The colonies of Xcc and Xanthomonas campestris pv. armoraciae are yellow, mucoid and surrounded by a zone of starch hydrolysis. For detailed information about Duchefa’s Phytopathological media have a look at our specialized brochure about this topic. Brochure can be found at the “Brochure section” at the home page of this website.
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| Filename | Language | File size |
|---|---|---|
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English | 334 KB |
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Nederlands | 340 KB |
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Deutsch | 339 KB |
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Français | 352 KB |
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Español | 348 KB |
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Italiano | 344 KB |
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Polish | 352 KB |
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Korean | 441 KB |
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Danish | 330 KB |
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Swedish | 331 KB |
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Finnish | 324 KB |
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Czech | 347 KB |
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Greek | 368 KB |
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Turkish | 340 KB |
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