B5 medium has been defined for the growth of cell suspensions of soybean root cells in the presence of 2,4 D. Nitrate was required in a concentration of 20-30 mM. An addition of 2 mM ammoniumsulphate led to an increase in cellgrowth. NH4+ when added as the sole source of nitrogen, did not support growth. Similar results were obtai-ned when NH4NO3 was substi-tuted for (NH4)2SO4. However, ammonium ions depressed growth when the concen-tration excee-ded 2 mM. Variati-ons in the concentrations of phosphate, calcium and magnesi-um resulted in relatively minor chan-ges in growth rate. Thiamine is known to be an essenti-al nutrient for cell growth and is increa-sed in concentrati-on up to 10 mg/l. B5 medium has been defined for the growth of cell suspensions of soybean root cells in the presence of 2,4 D. Nitrate was required in a concentration of 20-30 mM. An addition of 2 mM ammoniumsulphate led to an increase in cellgrowth. NH4+ when added as the sole source of nitrogen, did not support growth. Similar results were obtai-ned when NH4NO3 was substi-tuted for (NH4)2SO4. However, ammonium ions depressed growth when the concen-tration excee-ded 2 mM. Variati-ons in the concentrations of phosphate, calcium and magnesi-um resulted in relatively minor chan-ges in growth rate. Thiamine is known to be an essenti-al nutrient for cell growth and is increa-sed in concentrati-on up to 10 mg/l.
| Micro Elements |
mg/l |
µM |
| CoCl2.6H2O |
0.025 |
0.11 |
| CuSO4.5H2O |
0.025 |
0.10 |
| FeNaEDTA |
36.70 |
100.00 |
| H3BO3 |
3.00 |
48.52 |
| KI |
0.75 |
4.52 |
| MnSO4.H2O |
10.00 |
59.16 |
| Na2MoO4.2H2O |
0.25 |
1.03 |
| ZnSO4.7H2O |
2.00 |
6.96 |
| Macro Elements |
mg/l |
mM |
| CaCl2 |
113.23 |
1.02 |
| KNO3 |
2500.00 |
24.73 |
| MgSO4 |
121.56 |
1.01 |
| NaH2PO4 |
130.44 |
1.09 |
| (NH4)2SO4 |
134.00 |
1.01 |
| Vitamins |
mg/l |
µM |
| myo-Inositol |
100.00 |
554.94 |
| Nicotinic acid |
1.00 |
8.12 |
| Pyridoxine HCl |
1.00 |
4.86 |
| Thiamine HCl |
10.00 |
29.65 |
Total concentration Micro and Macro elements including -vitamins: 3163.98 mg/l
Gamborg O.L., Miller R.A., Ojima K., Nutrient requirement of suspensions cultures of soybean root cells. Exp. Cell Res., 50, 151 (1968).
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Hazard statements
- H315 Causes skin irritation
- H319 Causes serious eye irritation
- H335 May cause respiratory irritation
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Precautionary statements
- P261 Avoid breathing dust/fume/gas/mist/vapours/spray
- P302 IF ON SKIN:
- P305 IF IN EYES:
- P351 Rinse continuously with water for several minutes
- P352 Wash with soap and water
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01-2028
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01-2028
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10-2027
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Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
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G0210_P23634_GB.pdf |
P23634 |
Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
10-2027
Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
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Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
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Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
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G0210_P23319_GB.pdf |
P23319 |
Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
07-2027
Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
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Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
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Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
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G0210_P23320_GB.pdf |
P23320 |
Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
07-2027
Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
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Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
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Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
Warning: count(): Parameter must be an array or an object that implements Countable in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Db/Table/Abstract.php on line 1307
G0210_P22674_GB.pdf |
P22674 |
Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
01-2027
Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
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Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
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Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
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G0210_P22174_GB.pdf |
P22174 |
Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
09-2026
Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
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Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
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Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
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G0210_P21174_GB.pdf |
P21174 |
Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
09-2026
Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
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Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
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Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
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G0210_P22175_GB.pdf |
P22175 |
Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
09-2026
Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
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Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
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Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
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G0210_P21847_GB.pdf |
P21847 |
Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
06-2026
Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
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Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
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Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
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G0210_P21219_GB.pdf |
P21219 |
Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
02-2026
Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
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Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
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G0210_P21030_GB.pdf |
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Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
12-2025
Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
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Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
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G0210_P21029_GB.pdf |
P21029 |
Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
12-2025
Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
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