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Nitsch medium | Duchefa Biochemie

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N0223

Nitsch medium

The medium defined by Nitsch was used in the production of haploid plants of various species of Nicotiana raised from pollen grains. In this procedure, pollen that were still uninucleate were isolated and then cultured in vitro. Some pollen grains proliferate into embryo-like structures that develop in stages similar to those of zygotic embryos. The plantlets matured and flowered profusely, but did not set seed.

Micro Elements mg/l µM
CuSO4.5H2O 0.025
FeNaEDTA 36.70
H3BO3 10.00
MnSO4.H2O 18.94
Na2MoO4.2H2O 0.25
ZnSO4.7H2O 10.00
Macro Elements mg/l mM
CaCl2 166.00
KH2PO4 68.00
KNO3 950.00
MgSO4 90.27
NH4NO3 720.00

Nitsch J.P. and Nitsch C., Haploid plants from pollen grains, Science 169, 85 (1969). Nitsch J.P., Experimental androgenesis in Nicotiana, Phytomorphology 19, 389 (1969).


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Hazard statements

  • H315 Causes skin irritation
  • H319 Causes serious eye irritation
  • H335 May cause respiratory irritation
  • H412 Harmful to aquatic life with long lasting effects

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Precautionary statements

  • P261 Avoid breathing dust/fume/gas/mist/vapours/spray
  • P273 Avoid release to the environment
  • P302 IF ON SKIN:
  • P305 IF IN EYES:
  • P351 Rinse continuously with water for several minutes
  • P352 Wash with soap and water

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Filename Language File size

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N0223_GB.pdf
English 695 KB

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N0223_NL.pdf
Nederlands 704 KB

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N0223_DE.pdf
Deutsch 699 KB

Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206

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N0223_FR.pdf
Français 726 KB

Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206

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N0223_ES.pdf
Español 721 KB

Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206

Warning: count(): Parameter must be an array or an object that implements Countable in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Db/Table/Abstract.php on line 1307
N0223_IT.pdf
Italiano 715 KB

Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206

Warning: count(): Parameter must be an array or an object that implements Countable in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Db/Table/Abstract.php on line 1307
N0223_PL.pdf
Polish 727 KB

Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206

Warning: count(): Parameter must be an array or an object that implements Countable in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Db/Table/Abstract.php on line 1307
N0223_KO.pdf
Korean 825 KB

Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206

Warning: count(): Parameter must be an array or an object that implements Countable in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Db/Table/Abstract.php on line 1307
N0223_DA.pdf
Danish 695 KB

Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206

Warning: count(): Parameter must be an array or an object that implements Countable in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Db/Table/Abstract.php on line 1307
N0223_SV.pdf
Swedish 690 KB

Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206

Warning: count(): Parameter must be an array or an object that implements Countable in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Db/Table/Abstract.php on line 1307
N0223_FI.pdf
Finnish 685 KB

Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206

Warning: count(): Parameter must be an array or an object that implements Countable in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Db/Table/Abstract.php on line 1307
N0223_CS.pdf
Czech 719 KB

Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206

Warning: count(): Parameter must be an array or an object that implements Countable in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Db/Table/Abstract.php on line 1307
N0223_EL.pdf
Greek 746 KB

Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206

Warning: count(): Parameter must be an array or an object that implements Countable in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Db/Table/Abstract.php on line 1307
N0223_TR.pdf
Turkish 709 KB

Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206
  • N0223.0005

  • Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206

    Warning: count(): Parameter must be an array or an object that implements Countable in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Db/Table/Abstract.php on line 1307
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  • Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206

    Warning: count(): Parameter must be an array or an object that implements Countable in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Db/Table/Abstract.php on line 1307
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  • N0223.0025

  • Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206

    Warning: count(): Parameter must be an array or an object that implements Countable in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Db/Table/Abstract.php on line 1307
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  • Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206

    Warning: count(): Parameter must be an array or an object that implements Countable in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Db/Table/Abstract.php on line 1307
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Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206

Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206

Warning: count(): Parameter must be an array or an object that implements Countable in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Db/Table/Abstract.php on line 1307

Deprecated: array_key_exists(): Using array_key_exists() on objects is deprecated. Use isset() or property_exists() instead in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Registry.php on line 206

Warning: count(): Parameter must be an array or an object that implements Countable in /var/www/vhosts/duchefa-biochemie-old.bearwithus.nl/library/Zend/Db/Table/Abstract.php on line 1307